Mitochondrial plasmids of neurospora: Integration into mitochondrial DNA and evidence for reverse transcription in mitochondria
Identifieur interne : 004D50 ( Main/Exploration ); précédent : 004D49; suivant : 004D51Mitochondrial plasmids of neurospora: Integration into mitochondrial DNA and evidence for reverse transcription in mitochondria
Auteurs : Robert A. Akins [États-Unis] ; Robert L. Kelley [États-Unis] ; Alan M. Lambowitz [États-Unis]Source :
- Cell [ 0092-8674 ] ; 1986.
English descriptors
- Teeft :
- Aberrant growth, Agarose, Akins, Amino, Amino acid, Asterisk, Bertrand, Bglll, Biol, Clone, Conventional stopper mutants, Crassa, Cytochrome, Defective mtdnas, Deletion, Dna, Ecorl, Ecorl fragments, Experimental procedures, Fragment, Growth mutants, Growth variants, Hybridization, Hybridized, Insertion, Integration events, Intron, Junction fragment, Junction fragments, Lambowitz, Mauriceville, Mauriceville plasmid, Mitochondrial, Mitochondrial plasmid, Mitochondrial plasmid sequences, Mitochondrial plasmids, Mitochondrion, Mobile elements, Mtdna, Mtdna restriction fragments, Mtdna sequences, Mtdnas, Mutant, Nargang, Neurospora, Nucleotide, Plasmid, Plasmid sequences, Plasmid transcript, Plasmid transcripts, Primer, Putative, Race tubes, Rajbhandary, Reading frame, Recombinant, Recombination, Restriction fragments, Rrna, Sequencing, Subculture, Suppressive, Suppressive dnas, Suppressive plasmids, Transcriptase, Trna, Unpublished results, Variant, Variant plasmids, Varkud, Varkud plasmid, Varkud plasmids, Varkud strain, Varkud strains, Vries.
Abstract
Abstract: The Mauriceville (3.6 kb) and Varkud (3.8 kb) mitochondrial plasmids of Neurospora are closely related, closed circular DNAs whose nucleotide sequences and genetic organization suggest relationships to mitochondrial introns and retrotransposons. Here we isolated mutants whose growth is impaired as a result of malevolent behavior of these plasmids. All 12 mutants contain variant plasmids that are suppressive relative to mtDNA, and ten also contain defective mtDNAs. All the suppressive plasmids contain small insertions, generally including a mitochondrial tRNA sequence, at or near the major 5′ RNA start site. The structure of the suppressive plasmids suggests that they were generated via an RNA intermediate and a reverse transcription step. At least three of the mutants contain defective mtDNAs into which mitochondrial plasmid sequences have integrated. Sequences at the plasmid-mtDNA junctions are also consistent with integration via an RNA intermediate.
Url:
DOI: 10.1016/0092-8674(86)90615-X
Affiliations:
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Akins</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Genetics College of Biological Sciences Ohio State University Columbus</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Biochemistry College of Biological Sciences Ohio State University Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Kelley, Robert L" sort="Kelley, Robert L" uniqKey="Kelley R" first="Robert L." last="Kelley">Robert L. Kelley</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Genetics College of Biological Sciences Ohio State University Columbus</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Biochemistry College of Biological Sciences Ohio State University Columbus</wicri:cityArea></affiliation></author><author><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Genetics College of Biological Sciences Ohio State University Columbus</wicri:cityArea></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Ohio</region></placeName><wicri:cityArea>Department of Biochemistry College of Biological Sciences Ohio State University Columbus</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Cell</title><title level="j" type="abbrev">CELL</title><idno type="ISSN">0092-8674</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1986">1986</date><biblScope unit="volume">47</biblScope><biblScope unit="issue">4</biblScope><biblScope unit="page" from="505">505</biblScope><biblScope unit="page" to="516">516</biblScope></imprint><idno type="ISSN">0092-8674</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0092-8674</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Aberrant growth</term><term>Agarose</term><term>Akins</term><term>Amino</term><term>Amino acid</term><term>Asterisk</term><term>Bertrand</term><term>Bglll</term><term>Biol</term><term>Clone</term><term>Conventional stopper mutants</term><term>Crassa</term><term>Cytochrome</term><term>Defective mtdnas</term><term>Deletion</term><term>Dna</term><term>Ecorl</term><term>Ecorl fragments</term><term>Experimental procedures</term><term>Fragment</term><term>Growth mutants</term><term>Growth variants</term><term>Hybridization</term><term>Hybridized</term><term>Insertion</term><term>Integration events</term><term>Intron</term><term>Junction fragment</term><term>Junction fragments</term><term>Lambowitz</term><term>Mauriceville</term><term>Mauriceville plasmid</term><term>Mitochondrial</term><term>Mitochondrial plasmid</term><term>Mitochondrial plasmid sequences</term><term>Mitochondrial plasmids</term><term>Mitochondrion</term><term>Mobile elements</term><term>Mtdna</term><term>Mtdna restriction fragments</term><term>Mtdna sequences</term><term>Mtdnas</term><term>Mutant</term><term>Nargang</term><term>Neurospora</term><term>Nucleotide</term><term>Plasmid</term><term>Plasmid sequences</term><term>Plasmid transcript</term><term>Plasmid transcripts</term><term>Primer</term><term>Putative</term><term>Race tubes</term><term>Rajbhandary</term><term>Reading frame</term><term>Recombinant</term><term>Recombination</term><term>Restriction fragments</term><term>Rrna</term><term>Sequencing</term><term>Subculture</term><term>Suppressive</term><term>Suppressive dnas</term><term>Suppressive plasmids</term><term>Transcriptase</term><term>Trna</term><term>Unpublished results</term><term>Variant</term><term>Variant plasmids</term><term>Varkud</term><term>Varkud plasmid</term><term>Varkud plasmids</term><term>Varkud strain</term><term>Varkud strains</term><term>Vries</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The Mauriceville (3.6 kb) and Varkud (3.8 kb) mitochondrial plasmids of Neurospora are closely related, closed circular DNAs whose nucleotide sequences and genetic organization suggest relationships to mitochondrial introns and retrotransposons. Here we isolated mutants whose growth is impaired as a result of malevolent behavior of these plasmids. All 12 mutants contain variant plasmids that are suppressive relative to mtDNA, and ten also contain defective mtDNAs. All the suppressive plasmids contain small insertions, generally including a mitochondrial tRNA sequence, at or near the major 5′ RNA start site. The structure of the suppressive plasmids suggests that they were generated via an RNA intermediate and a reverse transcription step. At least three of the mutants contain defective mtDNAs into which mitochondrial plasmid sequences have integrated. Sequences at the plasmid-mtDNA junctions are also consistent with integration via an RNA intermediate.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Ohio</li></region></list><tree><country name="États-Unis"><region name="Ohio"><name sortKey="Akins, Robert A" sort="Akins, Robert A" uniqKey="Akins R" first="Robert A." last="Akins">Robert A. Akins</name></region><name sortKey="Akins, Robert A" sort="Akins, Robert A" uniqKey="Akins R" first="Robert A." last="Akins">Robert A. Akins</name><name sortKey="Kelley, Robert L" sort="Kelley, Robert L" uniqKey="Kelley R" first="Robert L." last="Kelley">Robert L. Kelley</name><name sortKey="Kelley, Robert L" sort="Kelley, Robert L" uniqKey="Kelley R" first="Robert L." last="Kelley">Robert L. Kelley</name><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name><name sortKey="Lambowitz, Alan M" sort="Lambowitz, Alan M" uniqKey="Lambowitz A" first="Alan M." last="Lambowitz">Alan M. Lambowitz</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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